The aim of this study was to develop an instructional instrument for biology
based on Religion, Environment, Society, and Technology (REST). It employed a bioentrepreneurship approach, described students’ creativity and interested in entrepreneurship while they were attending in the subject of environmental pollutions, which was a part of the environmental science. The subjects of this research were the second semester of students at IKIP PGRI Semarang, Biology Education Department. This research used a Research & Development method. The REST-based instructional instrument, helped by the bio-entrepreneurship approach, consisted of lesson plan, lectured contracts, modules, students’ work sheets, could be applied in the sub-subject of environmental knowledge. The research showed the creativity and entrepreneurship of the subject could be fostered by having them utilize many existing objects in the nearby surroundings, and experimental group was higher than control group.

Keywords: REST (Religion, Environment, Science and Technology),
Bioentrepreneurship, Environmental Knowledge.